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Abstract

TO CARRY OUT THE HEPATOPROTECTIVE EFFECTS ON LEAF OF DISTIMAKE DISSECTUS

R. Nagaraju*, V. Venkatesh Goud, P. Kamalakar, K. Kalpana, P. Srinivasulu,
A. Shivakumar and S. Venkataiah

ABSTRACT

The study reveals hepatoprotective (Dulbecco's modifiedEagles medium), MTT [3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide], trypsin, EDTA PhosphateBuffered Saline (PBS) and were purchased from SigmaChemicals Co. (St. Louis, MO) and Fetal Bovine Serum (FBS)were purchased from Gibco. 25 cm 2 and 75 cm2 flask and 96well plated purchased from Eppendorf India.

Keywords: Dulbecco's modified Eagles medium MTT [3- (4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide], trypsin, EDTA Phosphate Buffered Saline (PBS) and were purchased from Sigma Chemicals Co.


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