Abstract

DETERMINATION OF DOLUTEGRAVIR IN BULK AND ITS FORMULATION BY USING SIMPLE UV- SPECTROSCOPY

M. Rama Krishna, B. Gowri Bai, Ch. S. V. Chandu, G. Vishnu, K. Nandini Devi,
L. Jeevani, J. N. Suresh Kumar

ABSTRACT

UV spectroscopic method was developed for the estimation of Dolutegravir in bulk and Formulation. The UV spectrum of Dolutegravir in methanol and water mixture showed λ max at 258nm. Beer’s law is valid in the concentration range of 5-30µg/ml. This method was validated for linearity, accuracy, precision, LOD and LOQ. The method has demonstrated excellent linearity over the range of 5-30µg/ml with regression equation y = 0.0446-0.0055 and regression correlation coefficient r²= 0.9997. Moreover, the method was found to be highly sensitive with LOD (0.3602μg/ml) and LOQ (0.10915μg/ml). Depending on results the given method can be successfully applied for assay of Dolutegravir in formulation.

Keywords: UV spectroscopy, ?max, Linearity, Standard deviation, Relative standard deviation.