Abstract

ISOLATION AND IDENTIFICATION OF MICROBES FROM KITCHEN CLOTHS AND MENU CARDS OF DIFFERENT HOTELS

Krishnaveni R.*, Subitha S., Nithya M., Sabinagowri R., Mahalakshmi S., Sivapriya S., Lavanya M., Fowsal Mahira H., Fathima Mary S.

ABSTRACT

Kitchen cloths and menu cards in hotels can harbor pathogenic microbes, posing health risks. This study isolated and identified microbes from these surfaces in different hotels and tested their antimicrobial susceptibility. Bacteria like E.coli Staphylococcus aureus and Pseudomonas sp. were isolated. The findings highlight the need for improved hygiene practices in hotels to prevent foodborne illnesses. Microbial contamination of hotel menu cards and kitchen clothes poses potential health risks, as these items are frequently handled and often come into contact with food. This study aimed to isolate and identify microbes from these sources to assess hygiene levels. Swab samples were collected from menu cards and kitchen clothes in a local hotel and cultured on nutrient agar and MacConkey agar media. Bacterial isolates were characterized using Gram staining, colony morphology, and biochemical tests (catalase, oxidase, API kits). Results showed presence of Staphylococcus aureus, E.coli, Bacillus sp. and other bacteria, indicating potential hygiene concerns. Staphylococcus aureus, was predominant on menu cards, while E. coli was frequently isolated from kitchen clothes, suggesting possible fecal contamination. Antibiotic susceptibility testing revealed varying resistance patterns among isolates. The findings highlight the need for regular sanitation and hygiene monitoring of hotel items to prevent potential transmission of pathogens to patrons and staff.

Keywords: Staphylococcus aureus. E. coli, Pseudomonas sp, Antibiotic susceptibility, biochemical tests.