Abstract

EVALUATION OF INVITRO ANTIDIABETIC ACTIVITY OF SOME NOVEL SYNTHESIZED 2(5'- SUBSTITUTED PHENYL 1', 3’, 4’ OXADIAZOL-2'-YL) AMINO-6-FLUORO-7-SUBSTITUTED (1, 3)-BENZOTHIAZOLES DERIVATIVES

Ittagi Shanmukha*, Abhishek C. Pasarad, G. M. Sreenivasa, Vinuth Chikkamath, Chandana C. M.1, Anjali K. K.

ABSTRACT

Benzothiazole is a unique bicyclic ring system. The benzothiazole nucleus has a number of pharmacological effects, including antibacterial, analgesic, anticonvulsant, hypoglycemic, and anti-inflammatory activity. Oxadiazoles are recognized as a promising group of bioactive heterocycles. So, it is recognized as an important building block in the drug discovery and development process. According to a review of the literature, 1, 3, 4-oxadiazole is an important moiety for the development of new drugs among heterocyclic compounds. Compounds containing the 1,3,4-oxadiazole moiety have been shown to have a wide range of biological effects, including anticancer, antibacterial, antifungal, antihypertensive, antiviral, anti-HIV, and hypoglycemic properties. Anti-diabetic activity was assessed utilizing alpha-amylase inhibitory activity, with Acarbose as a standard reference. The absorbance was measured at 565 nm wavelength. The current study intends to examine the in-vitro alpha amylase inhibitory activity of several novel produced 2(5'-substituted phenyl 1', 3’, 4’ oxadiazol-2'-yl) amino-6-fluoro-7-substituted (1,3)- benzothiazole derivatives. The amylase inhibitory activity of the produced fluoro substituted benzothiazoles and oxadiazole derivatives B3, C3, F3, and V3 was investigated. In the alpha-amylase inhibition assay, a concentration of 7000 μg/ml resulted in maximal inhibition. As a result, our data suggest that B3, C3, and V3 may emerge as candidate molecules for anti-diabetic medicines, similar to the Acarbose effect.

Keywords: Benzothiazole, 1, 3, 4-oxadiazole, Anti-diabetic, alpha-amylase.