Abstract

NEW VALIDATED RP-HPLC MEHOD FOR THE DETERMINATION OF EPROSARTAN IN BULK AND PHARMACEUTICAL DOSAGE FORM

*Kamepalli Sujana, Medavankala Zabuda Vimmy Hamuthal

ABSTRACT

This investigation describes a new precise, sensitive and accurate RPHPLC method for the estimation of Eprosartan in bulk and pharmaceutical dosage forms.. The resolution of drug was achieved on symmetry c18 (150mm×4.6mm i.d.., 3µm particle size) column with UV detection at 235nm and mobile phase consists of sodium dihydrogen phosphate buffer PH 4.0 adjusted with ortho phosphoric acid and acetonitrile (40:60v/v). Using chromatographic conditions described Eprosartan was well resolved with mean retention time of 2.852min respectively. Linear response was observed over the range of 20-60µg/ml with a regression coefficient value 0.999. The lower limit of detection was found to be 9.95 and 2.95 for Eprosartan. The recovery results ranges in between 98% to 102% and the % RSD values were less than 2. The validation parameters were performed according to the ICH guidelines and the proposed method can be useful in the routine analysis for the determination of Eprosartan in pharmaceutical dosage forms.

Keywords: Eprosartan, Angiotensin II receptor antagonist, RP-HPLC, Symmetry column, validation parameters, TEVETEN.