Abstract

REVIEW ON HPLC METHOD DEVELOPMENT VALIDATION AND OPTIMISATION

*Mr. Dhrmesh Gangakachur, Ms. Abhilasha Nimkar, Ms. Tanushree Vaidya, Ms. Nargish Tembhurne, Adeshkumar Meshram

ABSTRACT

HPLC is the most popular method for separating, identifying, and quantifying compounds. Measurements are taken of drugs. The pharmaceutical industry is built upon the invention, development, and discovery of medicines. regarding the development and evaluation of analytical methods, such as the evaluation of pharmaceuticals the purity and toxicity of the substance. to enhance the HPLC techniques utilized to assess system performance. The study aims to look at a range of chromatographic factors. The method's improvement, refinement, and validation. The development of HPLC methods is contingent upon In addition to the pH and pKa values, the book also discusses the molecules' chemical makeup, polarity, solubility, and manufacturing process. the function of functional groups, and so on. The subject, categories, and applications are the primary topics of this review article.HPLC: applications, advantages, and disadvantages; process validation; equipment.

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