Abstract

HYPOGLYCEMIC AND ANTI-HYPERGLYCEMIC STUDY OF A POWDER COMBINATION FROM THREE LOCAL PLANTS

Simone Veronique FANNANG*, François EYA’ANE MEVA, Hans Denis BAMAL, NGENE Jean Pierre, Charles Christian Ngoule, Antoine Vayaraï Manaoda, Desline NKIANPI, Jean Emmanuel Mbosso Teinkela

ABSTRACT

Introduction: Hyperglycemia is defined as blood glucose > 140 mg/dl, and treatment is recommended when glucose levels are persistently > 140–180 mg/dl. Conventional therapies, including metformin, sulfonylureas, and insulin, have long served as the cornerstone of treatment. However, they often face limitations, such as adverse effects, reduced efficacy over time, The World Health Organization (WHO) has been playing a major role in the promotion of traditional medicine. This study aims to investigate the effect of a tri therapy of some local herbal resources (Allium sativum, Zingiber officinale and Hibiscus rosa sinensis), known for their anti hyperglycemic effects on glycemic control in laboratory rats. Materials and Methods: The plant materials for the study obtained from various sources were dried and crushed to powder then extracted in ethanol. This extract was concentrated and dried after which we obtained a fine powder to be used for diabetic control. The rats used in this study were reared in the animal laboratory. During the study, the rats were separated into six different groups each of which received 2g of glucose solution orally after monitoring their BGLs using the IVD glucose meter, followed by various substances per group (4 mg, 10 mg and 40 mg of powder combination, glimepiride2 and distilled water). The blood glucose levels of the rats in each group were taken at T0, T30, T60 and T90. The data obtained was analyzed using GraphPad Prism 8. Results: About 50 g of powder was obtained after drying the concentrated extract. The results of the analysis showed highly significant differences between the different groups at different times following the turkey multiple comparison test with p value 0.0034 between Group B (glimepiride2) and group E (powder at 40mg) , 0.0008 between group D (powder at 10mg) and E at T30. T30 being the focal point as the highest peaks of BGLs appeared at T30. Conclusion: The statistical analysis revealed the positive control (glimepiride2) to be the most active in glucose control. Powder combinations at 4mg and 10mg showed sharp peaks of hyperglycemia at T30 compared to other groups. The powder combination at 40mg showed normal glucose values but slightly high compared to the positive control. Thus the anti-hyperglycemic property of the powder combination was dose dependent, with 40mg being the most active relative to the other concentrations.

Keywords: Anti-hyperglycemic property, Hyperglycemic control, Glimepiride2.