Abstract

CRISPR AND BIOINFORMATICS: REVOLUTIONIZING GENOME EDITING THROUGH COMPUTATIONAL TOOLS

Manat Chakraborty, Somnath De*

ABSTRACT

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) has emerged as a transformative gene-editing platform, reshaping the landscape of modern molecular biology. The precision and success of CRISPR-mediated genome editing rely significantly on computational tools. These bioinformatics tools support the entire gene-editing workflow—from identifying target sequences and predicting off-target effects to designing guide RNAs (gRNAs) and performing post-editing analysis. This paper presents an overview of CRISPR technology, delves into the indispensable role of bioinformatics in optimizing CRISPR workflows, reviews prominent computational platforms, and highlights future prospects of this interdisciplinary synergy. Furthermore, the paper outlines the current limitations and proposes pathways to enhance accuracy and applicability, especially in medicine, agriculture, and biotechnology.

Keywords: CRISPR, bioinformatics, genome editing, gRNA design, off-target prediction, computational genomics