Abstract

ISOLATION AND IDENTIFICATION OF BIOACTIVE COMPOUNDS FOR PLANTS

Annu Kumari*, Rajaram R. Rajbhar*, Mr. Gopal Kumar

ABSTRACT

Plant extracts have long been used for their medicinal qualities and as sources of bioactive chemicals. In recent years, there has been a surge in interest in investigating the possibilities of plant extracts in a variety of disciplines, including medicine, cosmetics, and food. This abstract presents an up-to-date assessment of methods for extracting, isolating, and identifying bioactive chemicals from plant extracts, highlighting advances and problems in this field. The first stage in acquiring bioactive chemicals from plant sources is extraction. Maceration, infusion, decoction, solvent extraction, supercritical fluid extraction, and microwave-assisted extraction are among the extraction procedures used. These approaches strive to extract the target molecules as efficiently as possible while keeping their chemical integrity. Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug leads because of the unmatched availability of chemical diversity. Due to an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, interest particularly in edible plants has grown throughout the world. Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. . The analysis of bioactive compounds present in the plant extracts involving the applications of common phytochemical screening assays, chromatographic techniques such as HPLC and, TLC as well as non-chromatographic techniques such as immunoassay and Fourier Transform Infra-Red (FTIR) are discussed.

Keywords: Bioactive compound, Plant Extraction, Isolation, Herbal preparations, Natural products, Methods for extracting, identifying bioactive chemicals, Maceration, supercritical fluid extraction, microwave-assisted extraction.