GC-MS ANALYSIS AND "IN VITRO" ANTICANCER ACTIVITY OF METHANOLIC ROOT EXTRACT OF ASYSTASIA GANGETICA (L.) T.ANDERSON
Tamilselvan V.* Rajeswari M. and Velayutham P
ABSTRACT
The present study has been carried out to evaluate the phytochemical
constituents and "in vitro" anticancer potency of methanolic extract of
Asystasia gangetica (Acanthaceae). Phytochemical studies were done
by using Gas Chromatography- Mass Spectrometry analysis. In vitro
anticancer activity was evaluated with MTT standard colorimetric
assay against A549 human lung cell lines. Numerous novel anticancerous compounds including quercetin, isoquinoline, palladium,
lucenin, cervinomycin, ungeremine etc., have been identified from the
root extract paving way to do anticancer assay. "In vitro" MTT assay
of the root extract showed strong anti-proliferative effect with IC50
value of 106.2µg/ml to A549 human lung cell lines. The cytotoxic
effect was found to be concentration dependent. Increased concentration of the root extract
showed increased cytotoxicity.
Keywords: Asystasia gangetica, GC-MS, MTT Assay, A549 Human Lung Cell Line.
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