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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2020
Citation	6651	4087
h-index	26	21
i10-index	174	83

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WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

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WJPPS: NOVEMBER ISSUE PUBLISHED

NOVEMBER 2025 Issue has been successfully launched on 1 NOVEMBER 2025.

Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF GALLIC ACID AND PIPERINE IN HERBAL FORMULATION

Smita G. Patil*, Varsha M. Jadhav, Vilasrao J. Kadam

ABSTRACT

A simple, rapid and accurate spectrometric method based on solving simultaneous equation for simultaneous estimation of Gallic acid and piperine was developed. Gallic acid and piperine showed maximum absorbance at 273 and 343 nm respectively, absorbance was measured at these wavelengths for estimation of gallic acid and piperine respectively. Gallic acid and piperine both obeys beer Lambert's law in the concentration range of 2-12µg/ml. method was developed and validated as per ICH guidelines and can be applied for the routine estimation of the gallic acid and piperine in herbal formulation.

Keywords: Gallic acid, Piperine, Simultaneous equation, UV spectrophotometer, Validation.

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