Abstract

EVALUATION OF ANTIOXIDANT POTENTIAL OF CONJUGATED LINOLEIC ACID FROM DATURA INNOXIA MILL SEEDS USING ABTS AND FRAP ASSAYS

Dr. Veerendra S. Yadav*, Dr. Sanjay Gaikwad, Dr. Prasad Khandagale, Miss. Divya Kharat

ABSTRACT

Conjugated Linoleic Acid (CLA) is a bioactive fatty acid with documented health benefits, including potential anticancer properties. In this study, the antioxidant activity of CLA was evaluated using FRAP and ABTS assays to explore its relevance to cancer prevention and therapy. FRAP analysis revealed a progressive decline in absorbance with increasing concentration, stabilizing around 70% before a pronounced decrease between 90–100%, suggesting a concentration threshold influencing biological activity. The ABTS assay demonstrated a linear decline in absorbance, with CLA exhibiting higher values than Trolax, indicative of moderate aqueous-phase radical scavenging. However, the tested CLA formulation achieved markedly lower absorbance at high concentrations (≈0.09) compared to literature-reported values (0.18–0.25), signifyingenhanced scavenging potential. The observed antioxidant effects are particularly relevant to anticancer mechanisms, as oxidative stress contributes to carcinogenesis through DNA damage, inflammatory signaling, and apoptosis dysregulation. CLA’s ability to neutralize reactive oxygen species (ROS), modulate NF-κB–mediated inflammation, inhibit angiogenesis, and induce apoptosis underscores its dual antioxidant and anticancer potential. The sharp absorbance drop at high concentrations may reflect selective cytotoxicity toward cancer cells or oxidative degradation of active components, highlighting the need to optimize therapeutic dosage. These findings suggest that CLA, while less potent than Trolax in aqueous systems, may demonstrate amplified activity in lipid-rich tumor microenvironments, supporting its potential as a natural chemopreventive and adjunct therapeutic agent in oncology.

Keywords: Conjugated Linoleic Acid, FRAP, ABTS, antioxidant, anticancer, reactive oxygen species, Trolax, lipid peroxidation.