Abstract

BIODEGRADATION OF CICER ARIETINUM AMYLASE LOADED COCONUT OIL DRIVEN EMULSIFIED BOVINE SERUM ALBUMIN NANOPARTICLES AND THEIR APPLICATION IN WASHING DETERGENTS AS ECO-FRIENDLY BIO-ACTIVE ADDITIVE

Kirti Rani* and Chanchal Chauhan

ABSTRACT

In present work, Cicer arietinum amylase was encapsulated into coconut driven emulsified bovine serum albumin nanoparticle through glutaraldehyde. Biodegradation of coconut oil driven emulsified bovine serum albumin encapsulated Cicer arietinum amylase was carried out by using different units of alkaline protease (5U, 10U, 15U, 20U, 25U, 30U, 35U, 40U) to study their controlled and sustained release of encapsulated enzyme from prepared nanoparticles and hence, storage stability was enhanced up to 8 months as compared to free enzyme. Further, this coconut oil driven emulsified bovine serum albumin nanoparticles of encapsulated Cicer arietinum amylase with alkaline protease were used with detergents (aerial, surf excel, wheel and tide) for washing of stained cloth pieces which have coffee, tea, Pomegranate and turmeric stains. These chosen strains are very Common in our daily life and are very tough upon drying, hence, not to be easily washed off with well-known brand detergents upon in one wash. When, mixture solution of coconut oil driven emulsified bovine serum albumin nanoparticles of encapsulated Cicer arietinum amylase along with alkaline protease were used with chosen detergents powder for washing of different stained cloth pieces (coffee, tea, pomegranate and turmeric) leads to fast vanishing of these dry tough strains from cloth pieces with absolute clear visible results as compared to washing of stained cloths with detergents only.

Keywords: Cicer arietinum amylase, Bovine serum albumin, Glutaraldehyde, Encapsulated, Emulsified.