Abstract

SOME IN-SILICO AND IN-VITRO CONCEPTS ON CHIRAL THIN LAYER CHROMATOGRAPHIC SEPARATION OF SOME CHIRAL DRUGS AND DETERMINATION OF LEVOFLOXACIN IN PURE AND PHARMACEUTICAL DOSAGE FORMS

Yahya Abduh Salim Mohamed*, Tawfeek A. A. Yahya and Mohamed A. Alkhawlani

ABSTRACT

Some drugs have chiral centers that produce more than one enantiomer. One isomer is therapeutically active, thus this research concerned with the development of a new chiral thin layer chromatographic (CTLC) method for enantioseparation of some racemic drugs. The cited drugs are bisoprolol, chlorpheniramine, amlodipine and ofloxacin. The factors that play important role in chiral separation were studied as mobile phase composition, ratio of the mobile phase, chiral selector concentration, saturation time and pH. In addition, QSRR was done to determine 2D and 3D molecular descriptors that are influential in this mechanism. 2D molecular descriptors include rings, number of hydrogen bond acceptor atoms (a-acc), number of hydrogen bond donor atoms (a-don), molecular flexibility (KierFlex), third kappa shape index (Kier3), second alpha modified shape index (Kier A2), third alpha modified shape index (KierA3) and 3D descriptor that includes radius of gyration (rgyr).QSRR was modeled, evaluated and validated using MOE software. Furthermore, this method is used for analysis of levofloxacin in its pure and in pharmaceutical dosage forms with linearity range (100-1600 μg/mL) and the relationship between the drug concentration and the calculated peak area using Image J software was 0.996. LOD and LOQ were 25.24 and 75.72 μg/mL respectively.

Keywords: Chiral TLC, Amlodipine; Bisoprolol, Chlorpheniramine, Ofloxacin, Modeling, Levofloxacin, MOE, Image J.