Abstract

EVALUATION OF PHYTOCHEMICAL SCREENING AND THROMBOLYTIC ACTIVITY OF HYDRO ALCOHOLIC EXTRACT OF SOLANUM TORVUM FRUITS

Ushasree Pandiri, Nagu Maheshwari, Vadla Tejaswi, Roja Vedantham, Nooreen Unissa, Bojja Veerababu, Ragulapally Anitha and Bhukya Nagaraju*

ABSTRACT

Thrombosis is the formation of a blood clot (partial or complete blockage) within blood vessels, whether venous or arterial, limiting the natural flow of blood and resulting in clinical sequela. The ability of blood to flow freely in vessels relies on complex homeostasis that exists between blood cells (including platelets), plasma proteins, coagulation factors, inflammatory factors and cytokines, and the endothelial lining within the lumen of arteries and veins. When there is an imbalance with this physiologic process, there can be an increased risk of developing a thrombosis versus a coagulopathy (increased risk of bleeding). In certain clinical circumstances, patients can be at increased risk of thrombosis and bleeding simultaneously (e.g., disseminated intravascular coagulopathy-DIC, or in patients with underlying malignancy who develop a coagulopathy). As such, the diagnosis and management of thrombosis are complex. They can arise in any organ system, and their clinical presentation can vary depending on underlying comorbidities and presence (or absence) of provoking factors. Many factors can impact management decisions, including whether venous or arterial, acute or chronic, first or subsequent episode, family history, risk factor assessment, and hemodynamic stability. The extract of solanum torvum for thrombolytic activity and the results are presented in Table 1. Addition of 100µl SK, a positive control (30,000 I.U.), to the clots and subsequent incubation for 90 minutes at 37° C. showed 34.05% lysis of clot. On the other hand, distilled water was treated as negative control which exhibited a negligible. percentage of lysis of clot (13.63%). When clots were treated with 100 μι each of different concentrations (2 & 4mg/ml respectively) of the test sample significant clot lysis activity, i.e., 31.79% & 38.15 respectively, was observed and when compared with the negative control (water) the mean of percentage (%) of clot lysis was found most significant in case of 4mg/ml.

Keywords: coagulopathy, solanum torvum, lysis, intravascular coagulopathy-DIC, homeostasis.