Abstract

A REVIEW ON AFFINITY CHROMATOGRAPHY

Sangeetha S.*, Rajeev Krishnan B., Athira A. S. and Dr. Prasobh G. R.

ABSTRACT

Affinity chromatography is a type of liquid chromatography that uses a biologically related agent as a stationary phase to purify or analyse specific sample components. This chapter examines the basic components and principles of affinity chromatography and various applications of this method. The general types of ligands, immobilization schemes, support materials, and elution conditions that can be employed in affinity chromatography are first examined. Various methods in this field are then considered, including antibody purification, pull down assays, co-immuno precipitation, antigen purification using antibodies, avidin biotin system, recombinant tag protein purification, lectin affinity chromatography bio affinity chromatography. The types of ligands used in each of these methods are described, along with common applications of these techniques. Several related developments are also discussed, such as the use of affinity chromatography with mass spectrometry, miniaturized systems, molecularly imprinted polymers, and aptamers. The advantages and disadvantages of affinity chromatography are also included.

Keywords: The types of ligands used in each of these methods are described, along with common applications of these techniques.