Abstract

TOTAL SERUM IMMUNOGLOBULIN G LEVELS IN FEMALE WISTAR RATS (RATTUS NORVEGICUS) ADMINISTERED GEDI LEAF BREWING (ABELMOSCHUS MANIHOT)

Dingse Pandiangan*, Arby Nusa, Redsway Truman Douglas Maramis, Nelson Nainggolan, Pience Veralyn Maabuat and Michael Vallery Loeis Tumbol

ABSTRACT

Gedi leaf (Abelmoschus manihot) has been traditionally known for its health benefits, including its role as a natural immunomodulator. This study aimed to investigate the effect of Gedi leaf infusion on the serum Immunoglobulin G (IgG) levels in female Wistar rats (Rattus norvegicus). The research employed an experimental method with a completely randomized design, involving 10 female rats divided into two groups: 5 control rats (T0) as the comparison group and 5 treatment rats (Tp) per cage. The treatment group received Gedi leaf infusion for 34 consecutive days, while the control group was given only plain water without the infusion. The IgG levels were analyzed using the ELISA method. The results showed that the Immunoglobulin G levels in the treatment group (Tp) increased significantly compared to the control group (T0). The average IgG level in the control group (T0) was 194,286 ng/mL, while the treatment group (Tp) showed an increase to 942,640 ng/mL. The rise in immunoglobulin G after 34 days of treatment was 748,354 ng/mL. Statistical test results using Two-Sample t-Test Assuming Equal Variances showed a significant difference between control rats (T0) and treated rats (Tp). This study concludes that the content of Immunoglobulin G serum blood of female Wistar rats (Rattus norvegicus) during pregnancy to childbirth increased significantly after being given drinking Gedi (Abelmoschus manihot) brewed water for 34 days. The potential of Gedi brewed water to be used as an immunomodulator is very good for using Gedi leaf tea in the future, although research on other side effects is still needed.

Keywords: Gedi leaf, Immunoglobulin G, Wistar rats, ELISA.