Abstract

“ANTIOXIDANTS ACTIVITY OF MORIN HYDRATE AND CATECHIN HYDRATE, POTENTIAL ANTICANCER AGENTS”

Jagessar R.* and Roopchand J.

ABSTRACT

The antioxidant activity of two commercially available compounds: Morin hydrate (MH) & Catechin hydrate (CH) was evaluated using the DPPH (2, 2-diphenyl-1-picryhydrazyl) free radical scavenging assay. It was found that both compounds quench the DPPH signal at 517 nm. However, Morin hydrate seems to be a stronger antioxidant. The antioxidant activity was evaluated against a positive control in ascorbic acid. The latter was weaker in its antioxidant activity compared to Morin & Catechin. At 3ml dilution of stock Ascorbic acid solution, a 68.93% inhibition of DPPH in ethanol was observed compared to 97.73% and 98.3% inhibition induced by Catechin & Morin hydrate respectively. It was found that a 1ml dilution of stock solution of Morin & Catechin hydrate was sufficiently strong to quench the DPPH peak. Thus, a detailed version of the experiment was conducted and it was found that a 160uL and 170uL addition of Morin & Catechin hydrate respectively quenched the DPPH peak. Both Morin & Catechin hydrate were combined in a 1:1 mole ratio as it was found that the combination resulted in an increase in antioxidant activity as only 120uL were needed to quench DPPH radical max at 517nm. Thus, both compounds were found to be antioxidants and their future anticancer proficiency will be evaluated.

Keywords: antioxidant activity, Morin & Catechin hydrate, DPPH radical scavenging, anticancer.