Abstract

QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT EVALUATION, FRACTIONATION AND STRUCTURAL ELUCIDATION OF SOME INDIGENOUS PLANTS

Avinash Dhawade* and Sandeep Patel

ABSTRACT

Spermacoce hispida, commonly known as Shaggy Buttonweed & Spermacoce ocymoides, is a medicinal plant in the Rubiaceae family The plant has been used traditionally for various medicinal purposes, including treating nerve and kidney injuries, purifying blood, and improving vitality, according to tribal communities in Kerala. It's also being studied for its potential antidiabetic, antihypertensive, antioxidant, analgesic, anticancer, hepatoprotective, and antifungal properties The aim of this study is the quantitative phytochemical analysis, antioxidant evaluation, column chromatographic fractionation, TLC purification and isolation of fractions and GC-MS structural determination of isolated compounds. The aim of this study therefore is the bioassay guided extraction, fractionation purification to separate useful constituents and subsequent GC-MS structural elucidation of compounds present. The quantitative determination of the methanol extract was done using UV spectrophotometer for flavonoids,phenolics, alkaloids, tannins and saponins. The antioxidant activity was carried out using DPPH scavenging assay. The result shows that ethanol extract of Spermacoce hispida & Spermacoce ocymoides is a rich source of flavonoids, phenolics, alkaloids, tannins and saponins. Spermacoce hispida & Spermacoce ocymoides extract also show significant antioxidant activity (68%) which may be attributed to the synergistic effect of phytochemical content.

Keywords: Antioxidant, Phytochemical, Elucidation, Chromatography.