Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS: SEPTEMBER ISSUE PUBLISHED
  • SEPTEMBER 2021 Issue has been successfully launched on 1 September 2021.

Abstract

BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ESOMEPRAZOLE IN HUMAN PLASMA BY LC-MS/MS

SK.Razia Sulthana*, J.N.Suresh Kumar, V.Raghu Ram, Ajay Babu .Ch, N.Sambasivanaik

ABSTRACT

The present study aims at developing a simple, sensitive and specific liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for the quantification of Esomeprazole (EO) in human plasma using Omeprazole-d3 (OMD3) as an internal standard (IS). Chromatographic separation was performed on X-Bridge C18, 3.5μm, 4.6×50mm column with an isocratic mobile phase composed of 5mM Ammonium formate (pH 9.2) : Acetonitrile (65:35 v/v) at a flow-rate of 0.6 ml/min. EO and OMD3 were detected with proton adducts at m/z 346.1/198.3 and 349.20/198.0 in multiple reaction monitoring (MRM) positive mode respectively. EO and OMD3 were extracted by Precipitation method. The method was validated over a linear concentration range of 5.0-2000.0 ng/ml with a correlation coefficient of (r2) ≥ 0.9999. This method demonstrated intra and inter-day Precision within 1.6 to 2.3 and 2.0 to 2.2 % and Accuracy within 97.9 to 100.7 and 98.0 to 99.3 %. EO was found to be stable throughout freeze-thawing cycles, bench top and postoperative stability studies. This method was utilized successfully for the analysis of plasma samples following oral administration of EO (40 mg) in 27 healthy Indian male human volunteers under fasting conditions.

Keywords: Mass spectrometry; Precipitation method; Bioequivalence; Esomeprazole.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More