Abstract

A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ABACAVIR AND LAMIVUDINE IN PHARMACEUTICAL DOSAGE FORM

Dr. Madireddy Mamata* and Madhavi Reddy

ABSTRACT

A novel very rapid, sensitive, reverse phase High Performance Liquid Chromatography (RP-HPLC) technique was developed for the quantitative simultaneous estimation of Abacavir and Lamivudine in bulk and pharmaceutical dosage form. It was resolved by using a mobile phase of Phosphate Buffer, Methanol and Acetonitrile in the ratio of 65:25:10% v/v/v at a flow rate of 1.0 mL/min using UV-Visible detector at the wavelength of 260 nm for quantification. Efficient separation was achieved for Abacavir and Lamivudine on used Altima C18 (4.6mm × 150mm, 5.0 μm) Column. The retention times of Abacavir and Lamivudine were found to be 2.088 min and 6.068minutes respectively. The calibration graphs were linear and the method showed excellent recovery for Abacavir and Lamivudine respectively. The developed method was validated according to the International Conference on Harmonization (ICH) guidelines with respect to linearity, accuracy, precision, specificity and robustness. The results presented in this report revealed the development of simple, rapid, precise and accurate RP-HPLC method for immediatedetermination and validation of Abacavir and Lamivudine in bulk form and their marketed pharmaceutical dosage forms.

Keywords: Abacavir and Lamivudine, RP-HPLC, Accuracy, Precision, ICH Guidelines.