Abstract

EVALUATION OF COMBINED ANTI-INFLAMMATORY ACTIVITY OF PHLOROGLUCINOL AND ALPHA-LIPOIC ACID USING IN-VITRO AND IN-VIVO MODELS

K. Murugan*, Perumal Latha, Jayakanthan Parthasarathy, D. Padavi, M. Nariyo Chlebovanoa, R. O Nyarko, J. Yamoah and C. Asum

ABSTRACT

Background: Regardless of the particulars of the initial stimulus and the location of the reaction within the body, the pro-inflammatory response processes of an organism all share a fundamental mechanism that can be summed up as follows. This research work was performed to evaluate the anti-inflammatory response of the drugs that are phloroglucinol (PHG) and alpha-lipoic acid (ALA) in rats by combining them to form a more potent dosage with less side-effects as compared to other anti-inflammatory drugs that are already present in the market. Materials and method: In this research work anti-oxidant activity and anti-inflammatory potential of PHG, ALA and their combination was estimated through in-vitro methods. Moreover, anti-inflammatory activity was also evaluated through acute and chronic in-vivo rat models. Result: PHG and ALA combination had significantly produced anti-oxidant activity and markedly reduced 5-lipooxygenase (5-LOX) and cyclooxygenase (COX) level and also inhibited heat induced hemolysis in comparison to individual drugs as observed through in-vitro assays. The results of the in-vivo anti-inflammatory activity had also shown the promising therapeutic potential of PHG and ALA combination as observed through carrageenan induced paw edema and cotton pellet induced granuloma inflammatory models. Conclusion: Therefore, it can be concluded that combination of PHG and ALA has better therapeutic activity than individual drugs in the amelioration of inflammatory condition. Moreover, this increase in efficacy of combination may be due to the synergistic effect of both drugs.

Keywords: Phloroglucinol, Alpha lipoic acid, Inflammation, Granuloma, Carrageenan.