Abstract

GENOTOXICITY STUDY OF LAMBDACYHALOTHRIN IN WISTAR ALBINO RATS

Bhoopendra Kumar* and Nitesh Kumar

ABSTRACT

Genotoxicity study of lambda-cyhalothrin was conducted in wistar albino rats. The micronucleus and chromosomal aberration test were conducted @ dose level of 20 mg/kg b.wt. Whereas for bacterial reverse mutation test (Ames test) four dose levels 5, 100, 1000 and 5000 μg/plate were selected. The Salmonella typhimurium TA98, TA100, TA1535, TA97a and E. coli WP2 (uvra) bacterial strains respectively were used. The result indicated that lambda-cyhalothrin significant (p<0.05) increased micronucleus formation and PCE/NCE ratio in bone marrow. The chromosomal aberration test indicated significant (p<0.05) increase of aberrated bone marrow cells as chromatid break chromosome break, chromosome disc, sticky chromosome, satellite association and chromosome ring. However, lambda-cyhalothrin does not increase significant revertant colonies as compared to positive control group. Finally, it is concluded that lambda-cyhalothrin is genotoxic in wistar albino rats.

Keywords: lambda-cyhalothrin, micronucleus, chromosomal aberration, bacterial reverse mutation.