Abstract

BIOFILM AND ?-LACTAMASE (ESBL AND MBL) PRODUCTION IN PSEUDOMONAS AERUGINOSA ISOLATED FROM VARIOUS CLINICAL SAMPLES

Azhar Alam and Ravi Shankar Gupta*

ABSTRACT

Background: To assess Biofilm formation and β-lactamase production ability of Pseudomonas aeruginosa isolates. Methods: A hospital based cross-sectional study was conducted at National Medical College and Teaching Hospital, Birgunj, Nepal. The collection and processing of clinical samples were done following various standard microbiological techniques. Modified Kirby-Bauer disc diffusion method was followed to perform Antibiotic Susceptibility Test of the isolates. The phenotypic determination of Extended spectrum β-lactamase (ESBL) and Metallo β-lactamase (MBL) was done by combined disc method, and MIC testing was done by broth microdilution method. Detection of biofilm formation was done by micro-titer plate method. Results: The prevalence rate of P. aeruginosa in this study was found to be 23.01%. P. aeruginosa wasmostly isolated from pus sample. Amikacin (54.02%) and Meropenem (54.02%) showed greater susceptibility against P. aeruginosa isolates. Among 174 P. aeruginosa isolates, 72.41% were Multidrug resistant (MDR), 33.33% were Extended spectrum β-lactamase (ESBL) producers, and 45.97% were Metallo β-lactamase (MBL) producers. The MIC of Colistin showed 10.34% of colistin resistance among P. aeruginosa isolates. Biofilm formation was reported from 77.01% of isolates. This study witnessed positive correlation of MDR with biofilm formation, while the association of biofilm formation with ESBL and MBL production was found to be statistically insignificant. Conclusion: In conclusion, a significant proportion of P. aeruginosa isolates were reported to be MDR, ESBL producer

Keywords: AST, Biofilm formation, ?-lactamase, Pseudomonas aeruginosa, MDR.