Abstract

ISOLATION AND CHARACTERIZATION OF FLAVONE SUBSTITUTED BY METHOXY GROUPS FROM THE SHADE DRIED LEAVES OF CALOPHYLLUM INOPHYLLUM LINN

Christy K. Jose*, Francis Mathew and Santhosh M. Mathews

ABSTRACT

Plants used for traditional medicine include a vast range of substances that can be used to treat chronic as well as infectious diseases. The process of standardization may be achieved by stepwise pharmacognostic studies that includes organoleptic evaluation, microscopic evaluation, physicochemical standardization, qualitative phytochemical analysis and quantitative estimations of phytoconstituents. For the isolation of bioactive compounds a number of different separation techniques such as TLC, column chromatography, flash chromatography, Sephadex chromatography and HPLC are used to obtain pure compounds. The pure compounds so obtained are then used for the determination of structure and biological activity. The present study focusses on the isolation of pure phyto-constituents from the ethanolic extract of Calophyllum inophyllumleaves and its spectral characterization. Purification of bioactive compounds was done using column chromatography. The structure of the compound was elucidated on the basis of spectroscopic analysis, including Fourier transform infrared, electron spray ionization mass spectrophotometry, 1HNMR and 13CNMR. By the above spectral studies, the presence of a flavone substituted by methoxy groups at positions 5, 6, 7, 8, 3' and 4' respectively (Nobelitin) is predictedin the leaf tissue of Calophyllum inophyllum.

Keywords: Phytoconstituents, Calophyllum inophyllum, isolation, bioactive compound, spectral studies.