Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS: SEPTEMBER ISSUE PUBLISHED
  • SEPTEMBER 2021 Issue has been successfully launched on 1 September 2021.

Abstract

ENZYMATIC DEGRADATION OF GLIADIN BY NIGELLA SATIVA SEEDS PROTEASE: IMPLICATIONS FOR NEW TREATMENT OF CELIAC DISEASE

Nousseiba ABED*, Mohamed Nacer Bellir, Douadi Khlifi, Ines Bellil, Asma Medouri, Imane Medoukali, Leila Rouabah 

ABSTRACT

The protease was extracted from Nigella sativa seeds with 0,1 M citrate/phosphate buffer (pH 7,5), the crude enzyme extract showed maximum protease activity at pH 1,5 ,and optimal temperature at 50°C. After the partially purification of enzyme and analyses of RP-HPLC and SDS-PAGE results it appeared that Nigella sativa seeds protease degrade Triticum aestivum gliadin more efficiently than Triticum durum gliadin after 24h of incubation. The activity of Nigella sativa seeds protease with gliadin as substrate, in pH 7,5 at 37°C after 2h of incubation, before and after partial enzymatic purification prove that the crude enzyme extract have a low activity with Triticum durum gliadin however it was important with Triticum aestivum gliadin, this protease activity was increased in the same conditions using partially purified enzyme and it persist always higer with Triticum durum gliadin comparing with Triticum aestivum gliadin. On the bases of these results, Nigella sativa seeds protease represent the alternative means of treating celiac disease in the future using the detoxification of gliadin to eliminate the immunogenicity of gluten.

Keywords: Gliadin, Celiac disease, Nigella sativa, Protease.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More