Abstract

ISOLATION, CHARACTERIZATION AND IMMOBILIZATION OF SOIL MICRO ORGANISM

Leela Rani Bepala, Sahiti Nidhi Dhanvada, Sneha pinninti, Shiv Kiran Pinnada and
Sita Kumari Karanam*

ABSTRACT

Soil microorganisms are pivotal to ecosystem functioning, nutrient cycling, and environmental sustainability. This study investigates the isolation, characterization, and immobilization of soil microorganisms to evaluate their potential applications in agriculture, biotechnology, and environmental management. Serial dilution and plating techniques were employed for isolation, followed by Gram staining, motility assays, and IMViC tests for characterization. Key microbial traits, including morphology and metabolic activities, were identified. Additionally, microbial immobilization using sodium alginate gel was demonstrated to enhance microbial stability and functionality. These findings underscore the critical role of soil microorganisms in promoting sustainable agriculture, environmental remediation, and industrial processes.

Keywords: Soil microorganisms, isolation, characterization, immobilization, sodium alginate.