Abstract

BIOACTIVE PROPERTIES OF ENDOPHYTIC BACILLUS TEQUILENSIS (AUMC, B-500) STRAIN ISOLATED FROM CLOVE (SYZYGIUM AROMATICUM) PLANT

Safinaz J. Aburagaegah* and Alhadi M. Wajiej

ABSTRACT

A novel strain of endophytic bacterium, Bacillus tequilensis (AUMC, B-500) was successfully isolated from aromatic flower buds of the clove(Syzygium aromaticum) plant from Tripoli City, Libya. The bacterium's possible antioxidant and antibacterial properties were examined. The antibacterial activity of Bacillus tequilensis's ethyl acetate crude extract was evaluated against two common pathogenic bacterial strains: Staphylococcus aureus (ATCC®6538) and Streptococcus mutans(ATCC®25175). Furthermore, two tested pathogenic bacterial species demonstrated an in vitro growth inhibition in response to the ethyl acetate crude extract. Additionally, a DPPH radical scavenging assay was used to evaluate the antioxidant activity, and the recorded IC50 was 0.430 μg/mL. Furthermore, GC-MS was used to determine which compounds in the crude extract of bacterial ethyl acetate were effective. Nitro ethanol, Ethyl malonic acid, Pregn4-ene3,11,20-trione,6,17,21-tris [(trimethylsilyl) oxy], 3,20bis (Omethyloxime), (6á), and Ethanol amine are the most common compounds in the crude extract of ethyl acetate that are known to have antibacterial properties. The endophytic Bacillus tequilensis (AUMCB-500) was uploaded with the accession code OR775082 to GenBank. The identified isolate is a prospective strain with efficient metabolites. To the best of our knowledge, this study is the first to identify the endophytic Bacillus tequilensis (AUMC B-500) crude extract's antibacterial and antioxidant properties for possible use in the pharmaceutical drug industry.

Keywords: Bacillus tequilensis, Syzygium aromaticum, Streptococcus mutans, Endophytic bacteria, Bacterialmetabolites.