Abstract

ASSESSMENT OF ANTISTEROIDOGENETIC ACTIVITY IN FEMALE RATS THROUGH INVIVO AND BIOCHEMICAL ANALYSIS

D. Jothieswari* and G. Jayaprakash

ABSTRACT

The defatted methanol extract of Abelmoschus pungens was evaluated for its antisteroidogenic potential in adult female rats. The methanol extract at the dose of 250 and 500 mg /kg weight significantly. The vehicles and plant drugs were given orally in their proestrus on every over a period of 18 days. Body weight was noted and oestrus cycle was observed every day. A drop of normal saline was pipette in and out of the vagina and placed on a slide. The smear was stained with methylene blue and examined under the microscope. On 19th day, 18 hr fasting after the last dose and ovaries were dissected out and kept in cold 0.2 M sucrose buffer at room temperature. All the treated rats had corresponding recovery groups. At the end of each treatment periods, animal were killed and organ weight biochemical as well as ovarian easy enzyme were assessed. Elevated the levels of cholesterol and ascorbic acidcontents which serve as a precursor for the synthesis of steroid hormones in ovaries. The extract also significantly inhibited glucose-6-phosphate dehydrogenase and hydroxyl steroid dehydrogenase, the two key enzymes involved in ovarian steroidogenesis, protein content and also determination of oestrous cycle, body weight and histopathology. Hence the extract exhibited significant antisteroidogenic activity.

Keywords: Abelmoschus pungens: Antisteroidogenic activity: glucose-6-phosphate dehydrogenase, Protein content, Cholesterol, Ascorbic Acid and Histopathology.