Abstract

THERAPEUTIC EVALUATION OF PIMPINELLA GENUS PLANTS FOR WOUND HEALING

Samrudhi Santosh Patil*, Snehal Trimbak Daud, Sandip Rambhau Jadhav, Sima K. Tadvi and Asmi M. Kadam

ABSTRACT

The Pimpinella genus, known for its diverse phytochemical profile, has been traditionally used in folk medicine for various therapeutic applications, including wound healing. This study investigates the wound healing potential of selected Pimpinella species through both in vitro and in vivo evaluations. Extracts from different parts of the plant, including roots, stems, and leaves, were prepared using solvents of varying polarities to identify the most bioactive components. Phytochemical analysis revealed the presence of key compounds, such as flavonoids, tannins, and terpenoids, which are known to contribute to wound healing through anti-inflammatory, antimicrobial, and antioxidant mechanisms. The wound healing efficacy of these extracts was assessed using scratch assays for cellular migration andproliferation in fibroblasts, as well as animal wound models to monitor healing rates and tissue regeneration. The results demonstrated significant wound contraction, enhanced collagen deposition, and faster epithelialization in extract-treated groups compared to controls. Histopathological studies further confirmed reduced inflammation and improved tissue repair, indicating the effectiveness of Pimpinella extracts in promoting wound healing. This research suggests that Pimpinella species hold considerable potential as natural wound healing agents and warrant further exploration for the development of topical formulations aimed at enhancing skin repair and regeneration.

Keywords: Pimpinella genus, Wound healing, Herbal medicine, Phytochemicals, Anti-inflammatory, Antimicrobial activity, Antioxidant properties, Skin repair, Collagen deposition, Fibroblast proliferation.