Abstract

FORMULATION AND IN-VITRO EVALUATION OF NANO-EMULSION OF SERTRALINE

Chintala Bhanupriya*, Dr. M. Sunitha Reddy, Dr. K. A. Vijetha

ABSTRACT

Sertraline, a selective serotonin reuptake inhibitor, is the preferred medication for treating major depressive disorders and is one of the main therapeutic advancements in psychiatry. The aim of this study was to develop pharmaceutical nano emulsions and use lipid-based drug delivery systems (LBDDS) to increase the solubility of sertraline. By adjusting the ratio of surfactant (Tween 80) to co-surfactant (Transcutol P) and fixing oil (Capryol 90) and water, ternary phase diagrams were produced to detect Nano emulsion areas (Smix). After that, the chosen formulations were compared in order to optimize them. Based on optimization, three formulations—CT3T1(2:8), CT4T1(2:8), and CT4T1(3:7)—were chosen. A Smix ratio of 2:8 revealed a wider isotropic zone, and the Nano emulsion showed good stability. The CT3T1(2:8), CT4T1(2:8), and CT4T1(3:7) emulsions were successful in both centrifugation tests (3,500 rpm for 30 minutes), as well as the freeze-thaw cycle test (-20°C and +25°C), show that the emulsions remained intact under the applied stress conditions, showing no signs of phase separation, precipitation, or other discernible alterations. "CT3T1(2:8), CT4T1(2:8), and CT4T1(3:7) have polydispersibility index (PDI) values of 0.421, 0.398, and 0.431 for the first, second, and third months, respectively. These results show a relatively narrow and stable droplet size distribution. At the conclusion of the 6-hour Drug Release Kinetics testing period, CT4T1(2:8) had released 99.1% with a standard deviation of ±3.1%, which was much higher than the other two chosen (LBDDS) formulations. The stability of the Nano emulsions was assessed over a three-month period using CT4T1(2:8), which was selected for the accelerated stability experiments. The evaluation parameters and drug release behavior showed only slight variations after stabilization. In-vitro drug release studies, accelerated stability studies, droplet size, zeta potential, polydispersity index (PDI), scan spectrum, calibration curve, solubility studies, FTIR analysis, aqueous titration methods, evaluation of phase separation and centrifugation, thermodynamic stability studies, and more were all covered in the extensive evaluation of sertraline nano emulsions. Solubility profiles of different excipients, quantification techniques, calibration curve determination, and solubility studies were produced by the use of scan spectrum analysis. FTIR analysis assisted in determining functional groups and verifying the makeup of The formulations' potential for medicinal applications is highlighted by the nanoemulsions, which offer a solid basis for more study and advancement.

Keywords: LBDDS, Sertraline, Ternary phase diagram, Smix, Polydispersity index (PDI).