Abstract

STUDIES ON THE EVALUATION OF TERATOGENIC POTENTIAL OF AMINOGLYCOSIDE (AMIKACIN) ON DEVELOPING EMBRYOS OF GALLUS SP.: A BIOCHEMICAL STUDY

Tejas Sabnis* and Shalmalee Kulkarni

ABSTRACT

The teratogenic effect of the aminoglycoside antibiotic, Amikacin has been studied using chick embryos. This includes developmental malformations and alterations in enzyme biochemistry, notably Total protein content, alkaline phosphatase (ALP), and Acetylcholinesterase activity. It was observed that ALP (control: 91.22 IU/L, 100mM Amikacin: 47.83 IU/L) and Acetylcholinesterase (control: 942 IU/L, 100mM Amikacin: 355 IU/L) levels were markedly decreased after treatment with Amikacin. However, no significant difference was observed in total protein concentration in the control and treated embryos (control: 26.92 ug/ml, 100mM Amikacin: 23.97 ug/ml). These findings suggest that while Amikacin induces enzyme-specific disruptions, the broader protein landscape remains relatively unaffected. The study highlights the need for further research to elucidate the drug's effects on specific metabolic pathways leading to teratogenesis. From the above studies, whether Amikacin could be considered safe for administration during pregnancy remains elusive, and more work needs to be done on the same.

Keywords: Chick Embryo, Teratogenesis, Aminoglycoside, Amikacin, Alkaline Phosphatase (ALP), Acetylcholinesterase (AChE).