Abstract

PROCESS OPTIMIZATION FOR LIPASE PRODUCTION BY SOLID STATE FERMENTATION

Winee Surabhi Lall  Rekha Sirohi * and Veeru Prakash 

ABSTRACT

Microbial sources of lipase are cheaper, reliable and significant due to potential industrial applications. The aim of present study was to explore the production and optimization of lipase under solid state fermentation by psychrophilic bacterial strain Pseudomonas vancouverensis (JF508446.1) using cheaper sources of agro by-products i.e. linseed oil cake, rice bran oil cake, mustard oil cake, neem oil cake and their combinations as substrates. The results revealed that mustard oil cake is the best as lone source of substrate for lipase production but its combination with rice bran (1:1) resulted maximum activity (360 U/ml) at a time interval of 48 hrs. On addition of glucose and peptone to solid substrates, the production of lipase was found to be increased. Among different carbon sources supplemented, glucose (1% w/v) showed augmented lipase production (520 U/ml). Further, supplemented peptone (1% w/v) as the nitrogen source enhanced lipase production (480 U/ml). Partial purification of crude enzyme was done at different saturations of ammonium sulphate ranging from 30% - 60% and the maximum total activity and specific activity was obtained at 60% of saturation. Temperature of 15⁰C and a pH of 5.5 were observed most suitable for lipase activity under SSF.

Keywords: Lipase, Pscychrophillic, Pseudomonas vancouverensis, solid state fermentation, oil cakes, rice bran.