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Abstract

BASIC CONCEPTS IN UPLC – AN OVERVIEW

S. Palanichamy * P.Pavani, P.Ramasubramaniyan, M.Lakshmi, N.Sharanya, P. Solairaj

ABSTRACT

Ultra Performance Liquid Chromatography Systems take advantage of technological strides made in particle chemistry performance, system optimization, detector design, and data processing and control. When taken together, these achievements have created a step-function improvement in chromatographic performance. Defined as UPLC, this new category of analytical separation science retains the practicality and principles of HPLC while increasing the overall interlaced attributes of speed, sensitivity, and resolution. In this system uses fine particles (less than 2.5μm) so decreases the length of column, saves time and reduces solvent consumption.

Keywords: UPLC, HPLC, speed, sensitivity, resolution, column


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