Abstract

EVALUATION OF ANALGESIC ACTIVITY OF METHANOLIC EXTRACTS OF BOUGAINVILLEA SPECTABILIS STEM AND BARK IN SWISS ALBINO MICE

Hemank K.C., Laxmi Shahu, Rashmi Shrestha, Shivam Prasad Sah and Sanjeev Gurung*

ABSTRACT

Introduction: Pain is defined as "an unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms of such damage." Any member of the class of medications used to produce analgesia pain relief is referred to as an analgesic or painkiller. Objectives: To perform the evaluation of analgesic activity of methanolic extracts of Bougainvillea spectabilis stem and bark in Swiss Albino Mice. Methods: Soxhlets apparatus was used to extract the stem and bark of B.spectabilis. The extract was collected and orally administered (100 and 200 mg/kg). Central and peripheral analgesic activities of B.spectabilis were evaluated by hot plate, tail immersion and writhing test. Results: Using the hot plate method, it was discovered that there was a significant reduction in pain
with % reductions in analgesic activity of 3.74%, 9.74%, 9.44%, and 6.66% with 100 mg/kg extract administered, and 8.06%, 14.33%, 14.94%, and 12.62 % with 200 mg/kg extract administered. In the acetic acid induced pain method, the mean inhibition was 45.45 % (p < 0.002) for test 1 extract and 48.66 % (p < 0.001) for test 2 when compared to the control group. In tail immersion method, there was significant effect of high dose and low dose as compare to the standard at 30, 60, 90, 120 minutes. Conclusion: Our data indicates that B.spectabilis has a significant central and peripheral analgesic activity on both lower and higher dose.

Keywords: Bougainvillea spectabilis, Phytochemical screening, Hot plate method, Tail immersion method, writhing test.