Abstract

COLD-ADAPTED LIPASE PRODUCTION AND WASH PERFORMANCE EVALUATION BY KOCURIA ROSEA SSWC1

Syed Nisar Ahmed*

ABSTRACT

Psychrotrophic Kokuria rosae SSwC1, was isolated with the ability to produce extracellular, lipase from sweet curd obtained from dairy of Aurangabad. In terms of incubation time, agitation, substrate, pH, and temperature, the culture conditions for higher lipase production were optimized. After 5 days of incubation, a maximum lipase production of 35 U mL–1 was achieved in the medium at 200C and pH 8.0. The lipase was found to have excellent stability and consistency with commercial detergents, as well as a high efficiency for extracting stains at low temperatures. In contrast to detergent alone, the wash efficiency study lipase showed maximum reflectance of 12% was observed with Tide detergent and minimum of 9% with Ariel and Ghadi detergent for veg. oil stained fabric whereas maximum 12% reflectance for curd stained fabric with Ariel detergent and minimum of 7% with Nirma detergents.
These findings suggest that the supplementation of the lipase enzyme isolated from Kokuria rosae SSwC1in detergent could improve the removal of the veg. oil and curd stains at low temperatures.

Keywords: Psychrotroph, Kokuria rosae, alkaline lipase, enzyme activity, wash performance.