Abstract

CALOTROPIS GIGANTEA FLOWERS EXTRACTS WITH HIV-1 REVERSE TRANSCRIPTASE (RT) INHIBITORY ACTIVITY

Estari Mamidala*, Rajendra Prasad Gujjeti and Sainath Namthabad

ABSTRACT

The AIDS (Acquired Immunodeficiency Syndrome) in humans caused by the HIV-1 (Human immunodeficiency virus type 1) remains among the leading causes of death worldwide. In order to establish the study was undertaken to investigate the HIV-RT inhibitory activity of Calotropis gigantea flowers extracts. Sequential maceration method was performed for preparation of extracts using hexane, chloroform, ethyl acetate, acetone and methanol solvents. HIV-1 reverse transcriptase (RT) activity was done to assess anti-HIV activity of Calotropis gigantea flowers extracts. All crude extracts of Calotropis gigantea flowers showed significant inhibitory activity. Methanol extract exhibited the most notable activity of 86.5% against HIV-1 RT at 2mg/ml. Remaining chloroform, hexane, ethyl acetate and acetone extracts showed 86%, 85.6%, 85.6% and 82% respectively at 2mg/ml concentration. These findings suggested that Calotropis gigantea could be a potential source of phytochemicals having great importance as therapeutic agent.

Keywords: HIV-1, Reverse transcriptase, Calotropis giagantea, Flowers