Abstract

IDENTIFICATION AND EXTRACTION OF RIBOFLAVIN FROM COW’S MILK

Rajarshi Das, Priyanka Ray* and Sumana Roy

ABSTRACT

Riboflavin, also known as vitamin B2, is a vitamin found in food and sold as a dietary supplement. It is very important for formation of two major coenzymes, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). These coenzymes are involved in energy metabolism, and antibody production, as well as for normal growth and development of our body, especially for children. FMN and FAD are also required for the metabolism of vitamin B6 (Commonly called niacin), and vitamin B9 (Commonly called folate). Riboflavin, i.e., vitamin B2 is prescribed for treatment of corneal thinning, and also helpful in other diseases as it works as a protective against those diseases. We can use two extraction processes to extract the riboflavin- Acid extraction and Pepsin extraction. Milk contains three forms of riboflavin, with its free form being the most abundant. TLC with a chloroform-ethanol-acetone-concentrated ammonium hydroxide
mobile phase was used for the separation and identification of riboflavin in the extracts.

Keywords: Riboflavin, Vitamin B2, flavin, Acid extraction, Pepsin extraction, Milk.