Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF IBREXAFUNGERP BY RP-HPLC METHOD

*M. Bindu, M. Sreeja, S. Kusuma and Vishnu Priya

ABSTRACT

An Simple, sensitive, specific and precise RP-HPLC method for the pharmaceutical dose estimation of Ibrexafungerp. Chromatogram was run through AltimaC18 150 x 4.6 mm, 5. Mobile phase containing 0.1% DMSO(Dimethyl sulfoxide):Acetonitrile taken in the ratio 65:35was pumped through column at a flow rate of 0.9ml/min. Buffer used in this method was Dimethyl sulfoxide (DMSO) (4.2ph) buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 314.0nm. Retention time of Ibrexafungerp was found to be 2.584 min. %RSD of the Ibrexafungerp were and found to be 0.8. %RSD of Method precision of Ibrexafungerp was found to be 0.4. %Recovery was obtained as 99.42% for Ibrexafungerp. LOD, LOQ values obtained from regression equation of Ibrexafungerp were 0.13, 0.38. Regression equation of Ibrexafungerp is y = 43744x + 6926.8. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Keywords: Ibrexafungerp, RP-HPLC, DMSO, optimized wavelength, acetonitrile.