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Abstract

PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIMICROBIAL STUDIES OF MIRABILIS JALAPA AGAINST PATHOGENIC MICROORGANISMS

G. Harish*, Sandhya Rani M, B. Rajkamal, P. Mounika, M. Swetha.

ABSTRACT

The whole plant of Mirabilis jalapa was collected, pulverized, air dried and subjected to gradient extraction using soxhlation apparatus, dicholoro methane (DCM) was used as a solvent. Phytochemical screening revealed that the presence of alkaloids, reducing compounds (carbohydrates), glycosides, flavonoids, saponins, terpenes and steroids in moderate concentration. In vitro Antibacterial assay of the extract was preferred by agar nutrient broth dilution techniques. Plant extracts have shown broad spectrum activity on gram positive, negative organisms and no activity against fungal strain. The highest activity was shown by tubers extract with the MIC values of 12.5 μg/ml and 25 μg/ml against S.aureus and E.coli moderate activity was shown by stem extract with the MIC value of 100 and 25 μg/ml against S.aureus and E.coli and leaves extract shown least activity with the MIC values of 100 and 100μg/ml against S.aureus and E.coli. The tubers extract found to have a potent antimicrobial activity. This study provides some scientific base for use of this plant as a remedy for stomach, urinary tract, skin and microbial infections.

Keywords: Mirabilis jalapa, Whole plant extract, Phytochemicals, Antimicrobial activity.


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