Abstract

DEVELOPMENT AND VALIDATION OF UPLC METHOD FOR DETERMINATION OF ATENOLOL IN TABLETS FORM

P.Ravi Kumar Reddy*, V. Krishna Reddy, E.Sasikiran Goud

ABSTRACT

A simple, sensitive and accurate isocratic reverse phase ultra performance liquid chromatography method was developed for determination of atenolol in formulations. The effective separation was achieved on Zorbax-C18, 50x4.6mm with particle size 1.8μm. The buffer and methanol in the ratio 70:30v/v used as a mobile phase. The buffer was prepared as 1.1g of sodium-1-heptanesulfonate and 0.71g of anhydrous dibasic sodium phosphate in 700ml of water. Add 2ml of dibutyl amine and adjust the pH to 3.0 with 0.8 M phosphoric acid. Filter through 0.22μm filter and degas. The flow rate of the mobile phase was 1.0mL/min and the total elution time was 2 minutes. The UV detection wavelength was carried at 226nm and experiments were conducted at 25°C. The developed method was validated in terms of system suitability, selectivity, linearity, precision, accuracy, limits of detection and quantification for determination of atenolol following the ICH guidelines.

Keywords: Atenolol, Method development, ICH and UPLC.