Abstract

STUDIES ON DETERMINATION OF NON-ENZYMATIC ANTIOXIDANT ACTIVITIES OF ALOE VERA L. LEAF EXTRACTS ON OXIDATIVE STRESS INDUCED IN VITRO ANIMAL MODEL

Neelofar Khanam* and G. K. Sharma

ABSTRACT

Oxidative stress occurs when the production of reactive oxygen species overrides the antioxidant capacity in the target cell, resulting in the damage of macromolecules such as lipids, nucleic acids and proteins, causing alterations in the target cell function and leading to cell death. Since ancient time Aloe vera L. are using in many herbal preparations as antioxidants. Aloe vera L. possesses an important role in absorbing and neutralizing oxidant induced free radicals. In current investigation the non-enzymatic antioxidant effects of in vivo grown and in vitro propagated Aloe vera L. whole leaves and only gel extracts against oxidative stress induced in vitro animal model were studied. Here in present study, we used in vitro animal models as an alternate of using live animals in laboratory to reduce the harm and pain to live animals during laboratory experiments. Results were showing that among the different samples of methanol extracts of in vivo and in vitro regenerated Aloe vera L. whole leaves and inner gel, the methanol
extracts with in vitro regenerated Aloe vera L. leaves were showing maximum protection against oxidative stress in in vitro animal model.

Keywords: Oxidative stress occurs when the production of reactive oxygen species overrides the antioxidant capacity in the target cell, resulting in the damage of macromolecules such as lipids, nucleic acids and proteins, causing alterations in the target cell func