Abstract

SIMULTANEOUS ESTIMATION OF QUINAPRIL AND HYDROCHLORTHIAZIDE IN TABLETS BY RP-HPLC METHOD

*Rama Prasad Padhy, K. Srinivas, Subas Chandra Dinda

ABSTRACT

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of quinapril and hydrochlorthiazide in tablets. A column having 250 x 4.6 mm, 5μ i.d. in gradient mode with mobile phase containing Methanol: Water (80:20) was used. The flow rate was 1.0 ml/min and effluent was monitored at 232 nm. The retention time (min) and linearity range (ppm) for quinapril and hydrochlorthiazide were (3.6, 2.2) and (20-80, 20-80), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of quinapril and hydrochlorothiazide in tablets.

Keywords: Quinapril, hydrochlorthiazide, RP-HPLC, simultaneous determination, tablets.