Abstract

PROTOCATECHUIC ACID AMELIORATES UUO-INDUCED RENAL INJURY IN MALE MICE VIA REDUCTION OF OXIDATIVE STRESS, INFLAMMATION AND FIBROSIS

Karim M. Saad, Évila Lopes Salles, Sahar Emami Naeini, Babak Baban, Marwa E. Abdelmageed, Rania R. Abdelaziz, Ghada M. Suddek and Ahmed A. Elmarakby*

ABSTRACT

Objectives: Chronic kidney disease (CKD) could result from obstructive nephropathy; a condition that arises from urinary flow obstruction in the kidney leading to renal inflammation and injury. Protocatechuic acid (PCA) is a natural phenolic compound that shows a promising protective effect in cardiovascular diseases. In the current study, the reno-protective effects of PCA were investigated in a mouse model of unilateral ureteral obstruction (UUO). Methods: Four groups of male C57BL/6J mice (10-12 weeks old) were used (n=6-8/group); Sham control, Sham/PCA (50 mg/kg I.P daily for two weeks), UUO and UUO/PCA. Results: PCA significantly improved the UUO-induced decline in creatinine clearance and excretion. Moreover, PCA attenuated renal TBARs, IL-6, matrix metalloproteinase-2 (MMP-2) and MMP-9 in UUO-induced renal injury. Furthermore, PCA also significantly attenuated UUO-induced increase in renal collagen IV and KIM-1 expression. Conclusion: These data suggest that PCA provides renal protection in murine model of UUO via reduction of renal oxidative stress, inflammation and fibrosis. 

Keywords: UUO; Kidney injury; Protocatechuic acid; Oxidative stress; Inflammation; fibrosis.