Abstract

EFFECT OF FERULIC ACID ON MYOCARDIAL TISSUE LEVELS OF TBARS, GSH, SOD, CATALASE AND TISSUE NITRATE IN RATS

Dr. Mekala Sunil*, Dr. S. Naga Subramanyam, A. Pravallika, Ch. Santhi Swaroopini, M. Pallavi, P. Navya and N. Sravani

ABSTRACT

The present study was designed to evaluate the cardioprotective role of Ferulic acid on the in vivo myocardial ischemic reperfusion injury in rats. Methods: Male Wistar albino rats were divided into six groups (n=6). The group C & C-IR animals were administered saline orally (sham, I-R Control group), animals group FA-BL1& FA-BL2 recevied ferulic acid 20mg/kg and 40mg/kg b.wt respectively upto 30 days orally without inducing I-R injury and animals group. FA-IR1 & FA-IR2 recevied FA 20mg/kg and 40mg/kg respectively upto 30 days. On the 30th day animals of C-IR, FA-IR1 & FA-IR2 groups were underwent 15 minutes of ligation of left anterior descending coronary artery and were thereafter reperfused by 60 minutes. Results: In the C- IR group, myocardial infarct size was observed by the presence of unstained necrotic tissue, a significant decrease in the levels of endogenous anti oxidant enzymes (GSH,CAT,SOD), tissue nitrate (NO), cardiac markers (LDH, CK, AST) and increased the lipid
peroxidation levels (TBARS) due to the myocardial damage by ischemic reperfusion injury. Chronic oral administration of FA at both doses (20mg/kg and 40mg/kg) significantly restored the myocardial anti oxidant status evidenced by increased SOD, CAT & GSH, tissue nitrate levels, diminished the TBARS levels and prevented the leakage of cardio-specific enzymes LDH,CK,AST. This is further confirmed by histopatholoical changes. Conclusion: Ferulic acid significantly attenuates myocardial ischemic reperfusion injury through augmentation of endogenous antioxidants, stabilization of myocardial membrane permeability and induction of nitric oxide production.

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