Abstract

ASSESSMENT OF CHRONIC ADMINISTRATION OF AMLODIPINE IN CEREBELLUM OF WISTAR RATS

Ponle Bamidele Fakunle*, Israel Oguns Ogunyemi and Samuel Ehireme

ABSTRACT

Hypertension, a prevalent cardiovascular condition, necessitates effective management through antihypertensive medications. Amlodipine, a widely prescribed calcium channel blocker, exhibits potent antihypertensive effects. However, its comprehensive impact on physiological parameters, especially the cerebellum, remains inadequately explored. We conducted an in-depth investigation into the impact of Amlodipine on various biological systems. Male Wistar rats were administered Amlodipine at three different doses (T1: 2.5mg/kg, T2: 5mg/kg, T3: 10mg/kg) for two weeks. Hematological indices, antioxidant defenses, and cerebellum morphology were meticulously examined. Our study revealed a significant increase in red blood cell (RBC) counts in the T2 and T3 groups, indicating a dose-dependent
effect on erythrocyte homeostasis. White blood cell (WBC) counts decreased significantly in all treatment groups, suggesting reduced systemic inflammatory response. Platelet counts exhibited a significant decrease, potentially impacting thrombopoiesis. Packed cell volume (PCV) increased notably in the T2 group, indicating alterations in blood composition. Serum total protein levels showed dosage-dependent fluctuations, with a significant increase in the T3 group. Superoxide dismutase (SOD) and catalase (CAT) levels signified improved antioxidant response and enzymatic defenses. Histological studies using Cresyl Violet and Hematoxylin and Eosin (H&E) staining revealed cerebellum alterations, emphasizing potential neurotoxic effects. Body, brain, and cerebellum weights varied across groups, highlighting the systemic impact of Amlodipine. Our findings underscore the multifaceted influence of Amlodipine on hematological indices, antioxidant defenses, and cerebellum morphology.

Keywords: Amlodipine, Hypertension, Hematological indices, Antioxidant enzymes, Cerebellum.