Abstract

STABILITY STUDY OF SHARPUNKHA VATI WITH RESPECT TO VARIOUS MICROBIAL DIAGNOSTIC MODALITIES

Dr. Naresh B. Chaudhary*, Dr. Nikita Patir, Dr. Meera S. Cholera and Dr. Virendra K. Kori

ABSTRACT

Background: In last few decades, market of herbal and traditional medicines has grown up in leaps and bounds, but majority of traditional medicines are lacking in its data of Saviryatavadhi (shelf-life period). Hence the present study was carried out to evaluate the stability of Sharpunkha Vati with respect to microbial contamination and fungal growth. Material and method: Sharpunkha Vati was prepared in the pharmacy ITRA on date 3rd June 2022 and stored in food grade air-tight plastic containers, in a well-ventilated, well lighted space at room temperature. A baseline microbial profile was studied at regular interval of 1 month for total 13 months. Every time, the sample was subjected to microbiological studies like smear examination with wet mount - 10% K.O.H. preparation and Gram’s stain; fungal culture;
and aerobic culture at Microbiology Laboratory, ITRA, Jamnagar. Results: In this study, no any growth of micro-organisms (bacterial or fungal) was found till 21st June 2023 i.e., for 13 months from the date of preparation. Conclusion: Hence this study shows that Sharpunkha Vati can remain stable in various climatic conditions for a minimum of 13 months and remain free from any microbial contamination, which indicate that the manufacturing and storage practices adopted in this study was up to the mark of Good Manufacturing Practice (GMP) and could be used as quality standards by future researchers and drug manufacturers.

Keywords: Microbial contamination, Sharpunkha Vati, Stability study, Climate condition.