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Alagar Raja.M*, Asma, David Banji, Rao.KNV, SelvaKumar.D


SPME is found to be a superior sample preparation technique targeting for organic components, including GSR analysis. Gas chromatography combined with varied detection systems performed prior to SPME gives low detection limits in simpler, easier and rapid manner. Solid-phase micro extraction, or SPME, is a sample preparation technique that involves the use of a fiber coated with an extracting phase, that can be a liquid (polymer) or a solid (sorbent), which extracts different kinds of analytes (including both volatile and non-volatile) from different kinds of media, that can be in liquid or gas phase. The quantity of analyte extracted by the fibre is proportional to its concentration in the sample as long as equilibrium is reached or, in case of short time pre-equilibrium, with help of convection or agitation. Pesticides are an important and diverse environmental and agricultural species. Their determination in formulations, in feed and food, and in complex environmental matrices (e.g., water, soil, sludge, sediments, etc.) often requires separation methods capable of high efficiency, unique selectivity, and high sensitivity. Because pesticides (organophosporus, organochlorine, carbamate, dithiocarbamate, etc.) are carcinogenic, they are problematic for humans in the course of the food chain. Residual analyses have been performed to find out the concentration and type of pesticides and their metabolites left in food at the time of consumption. This ultimately helps in production of better and human-friendly pesticides.

Keywords: SPME, GSR analysis, fiber coated environmental matrices.

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