HPTLC FINGER PRINTING ANALYSIS OF THE STEROIDS FROM PONGAMIA PINNATA LEAVES
Amita Tilak*, Mudit Kumar and Sudhir Singh Gangwar
ABSTRACT
Objective: The present study was conducted to identify the Steroids from ethanol extract of medicinally and economically useful leaves of Pongamia pinnata using. High Performance Thin Layer Chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V. applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of
tungstant lamp. Results: HPTLC finger printing of Steroids of ethanol extract of leaves revealed Different Rf showing fluorescence hence steroids are detected. Conclusions: With the results of HPTLC analysis Steroids have been concluded in the extract.
Keywords: Pongamia pinnata leaves, Steroids, HPTLC Fingerprinting.
[Download Article]
[Download Certifiate]