Abstract

SYNTHESIS AND SCREENING OF NEWER SEMICARBAZIDE DERIVATIVES FOR ITS ANTI-NOCICEPTIVE EFFECT

*Swati Pathak and Abhinav Prasoon Mishra

ABSTRACT

The present study was based on the synthesis and screening of newer semicarbazide derivatives for its anti-nociceptive effect using animal models. Due to their vast range of biological actions and role as building blocks in many different classes of significant pharmacological compounds, semicarbazide derivatives have attracted a lot of attention. In the presence of ethyl alcohol, semi-carbazide is made react at high temp. with substituted benzaldehyde thus received the intermediate I that is further made reacted with acetic acid to get the final product. Thus, semicarbazide derivatives were synthesized, characterized for melting point, TLC, FTIR and NMR spectroscopy and evaluated their anti-nociceptive activity using following parameters like hot-plate method and tail-flick method. Rats (both sexes) weighing between 120 and 150g were supplied by Animal House, Advance Institute of Biotech & Paramedical Sciences, Kanpur. The rats are kept in ideal conditions, with a room temperature of 25°C and a light/dark cycle of 12 hours every day. Animals were divided into 4 groups i.e., group 1 administered normal saline, group 2 administered diclofenac (50mg/kg, i. p.), group 3 and group 4 administered all the newer derivatives of semi-carbazide (at dose of 100mg/kg, i. p. and 200mg/kg, i. p.), respectively. In results, all the derivatives of synthesized semi-carbazides were shown analgesic potential when tested at 100mg/kg and 200mg/kg. They significantly increased the basal reaction time at diverse time intervals and activity was in dose-dependent manner. Analgesic action might be due to blockade of inflammatory mediators like interleukins, thromboxane, cytokines etc. So, it might be effective in the management of pain subtypes. It might be given in central or peripheral pain which is most common symptom of many medical conditions or injury. It would be cost-effective with easy availability among society. It refers to determine the mode of action that how semi-carbazide derivatives subside pain whether its internal or external one.

Keywords: Semi-carbazide, synthesis, characterization, anti-nociceptive, tail flick method.